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2015
Susin, C, Wagner MC, Haas AN, Oppermann RV, Albandar JM.  2015.  The association between alcohol consumption and periodontitis in southern Brazilian adults, Oct. J Periodontal Res. 50:622-8., Number 5 AbstractWebsite

Susin, CWagner, M CHaas, A NOppermann, R VAlbandar, J MengResearch Support, Non-U.S. Gov'tJ Periodontal Res. 2015 Oct;50(5):622-8. doi: 10.1111/jre.12242. Epub 2014 Nov 17.

BACKGROUND AND OBJECTIVE: An association between alcohol consumption and periodontitis has been suggested in the literature, but the evidence is still unclear. The aim of the present study was to investigate the relationship between alcohol consumption and periodontitis in a probability sample of adults from south Brazil. MATERIAL AND METHODS: This analysis included 1115 subjects aged 18-65 years derived from a representative sample from south Brazil. Data were collected from participants from clinical examination and structured interviews. Alcohol consumption was assessed by asking participants about the usual number of drinks consumed in a week. Four categories of alcohol consumption were defined: non-drinker, 1 glass/wk, 1 glass/d. Individuals with >/= 30% teeth with periodontal attachment loss >/= 5 mm were classified as having periodontitis. Logistic models adjusting for age, race, socioeconomic status, dental care, body mass index, self-reported diabetes and smoking were used to estimate odds ratios (OR) and confidence intervals (95% CI). RESULTS: After adjusting for co-factors, females who reported drinking > 1 glass/d were more likely to have periodontitis (OR = 3.8, 95% CI = 1.4-10.1), whereas females who reported drinking up to 1 glass/d were 50% less likely to have periodontitis (OR = 0.5, 95% CI = 0.3-0.8). No significant associations between overall alcohol intake and periodontitis were observed for males. In an exploratory analysis, wine consumption was associated with a lower likelihood of periodontitis among males (OR = 0.2, 95% CI = 0.1-0.5) but not females. CONCLUSION: The periodontal health of males and females appears to be affected differently by alcohol consumption. Moderate wine consumption may have a beneficial effect in males.

Franzon, R, Opdam NJ, Guimaraes LF, Demarco FF, Casagrande L, Haas AN, Araujo FB.  2015.  Randomized controlled clinical trial of the 24-months survival of composite resin restorations after one-step incomplete and complete excavation on primary teeth, Oct. J Dent. 43:1235-41., Number 10 AbstractWebsite

Franzon, ROpdam, N JGuimaraes, L FDemarco, F FCasagrande, LHaas, A NAraujo, F BengRandomized Controlled TrialEnglandJ Dent. 2015 Oct;43(10):1235-41. doi: 10.1016/j.jdent.2015.07.011. Epub 2015 Jul 29.

OBJECTIVE: This randomized clinical trial aimed to compare the 24-months survival of composite restorations in primary molars after partial caries removal (PCR) and total caries removal (TCR). METHODS: Forty-eight children aged 3-8 years with at least one molar with a deep carious lesion were included (PCR; n=66; TCR; n=54). For PCR, excavation was stopped when dentine with a leathery consistency was achieved; in the TCR group, total absence of carious tissue was confirmed using a blunt-tipped probe. Pulpotomy was performed in cases of pulp exposure. Success was assessed by modified USPHS criteria with Alpha and Bravo scores recorded as success. RESULTS: Pulp exposure occurred in 1 and 15 of the teeth treated with PCR and TCR respectively (p<0.01). The restorations survival rate after 24 months was 66% (PCR) and 86% (TCR) (p=0.03). When teeth that received pulpotomy were analyzed separately, the survival rate was 92% (p=0.09). PCR performed in occlusoproximal restorations demonstrated the lowest success rate (p=0.002). PCR increases 2.90 times the probability of having a restorative failure compared to TCR (p=0.03), after adjusting for cavity type. When pulp exposure and restoration failure were considered as the outcome, there was no significant difference between the two groups (p=0.10) with success rates of 64% (PCR) and 61% (TCR). CONCLUSION: Collectively, deciduous teeth submitted to PCR prevented pulp exposure and, consequently, more invasive treatments; otherwise, PCR yielded lower longevity for composite restoration compared to TCR, suggesting that PCR restorations need to be followed over time, especially when multi-surface restorations are involved. CLINICAL SIGNIFICANCE: Composite restorations on carious remaining tissue require monitoring over time, especially those performed in more than one surface. Even if the restorations present shortcomings over the time, the majority of them are subject to repair, allowing more conservative approaches for teeth with deep caries lesions.

Saffi, MA, Furtado MV, Polanczyk CA, Montenegro MM, Ribeiro IW, Kampits C, Haas AN, Rosing CK, Rabelo-Silva ER.  2015.  Relationship between vascular endothelium and periodontal disease in atherosclerotic lesions: Review article, Jan 26. World J Cardiol. 7:26-30., Number 1 AbstractWebsite

Saffi, Marco Aurelio LumertzFurtado, Mariana VargasPolanczyk, Carisi AnneMontenegro, Marlon MunhozRibeiro, Ingrid Webb JosephsonKampits, CassioHaas, Alex NogueiraRosing, Cassiano KuchenbeckerRabelo-Silva, Eneida RejaneengReviewWorld J Cardiol. 2015 Jan 26;7(1):26-30. doi: 10.4330/wjc.v7.i1.26.

Inflammation and endothelial dysfunction are linked to the pathogenesis of atherosclerotic disease. Recent studies suggest that periodontal infection and the ensuing increase in the levels of inflammatory markers may be associated with myocardial infarction, peripheral vascular disease and cerebrovascular disease. The present article aimed at reviewing contemporary data on the pathophysiology of vascular endothelium and its association with periodontitis in the scenario of cardiovascular disease.

Oppermann, RV, Haas AN, Rosing CK, Susin C.  2015.  Epidemiology of periodontal diseases in adults from Latin America, Feb. Periodontol 2000. 67:13-33., Number 1 AbstractWebsite

Oppermann, Rui VHaas, Alex NRosing, Cassiano KuchenbeckerSusin, CristianoengReviewDenmarkPeriodontol 2000. 2015 Feb;67(1):13-33. doi: 10.1111/prd.12061.

A decade has passed since we first reviewed the epidemiology of periodontal diseases in Latin America. At that time, lack of population-based studies was the norm and our conclusions were based on very limited evidence. The aim of the present comprehensive review was to update and expand our previous work by providing a broad overview of Latin America and its current social, economic and demographic status and by focusing on the epidemiology of periodontal diseases in Latin American adults published in the last 15 years. The amount of periodontal epidemiological data available has increased but is still restricted to a few countries only. The scope of the literature available has also broadened to include oral health-related quality of life and systemic interactions; however, most studies had methodological limitations that might have biased their results. In general, periodontitis was very prevalent, but severe periodontal destruction was localized. Besides being associated with well-established risk factors, periodontitis was associated with demographics and socio-economic factors in Latin American populations. To advance epidemiological knowledge, population-based cross-sectional and longitudinal studies, using appropriate methodologies, should be the future focus of the research agenda of researchers and public health planners in Latin American countries.

Haas, AN, Gaio EJ, Wagner MC, Rios FS, Costa Rdos S, Rosing CK, Oppermann RV, Albandar J, Susin C.  2015.  A population-based cohort study of oral health in South Brazil: The Porto Alegre Study, Apr-Jun. Rev Bras Epidemiol. 18:515-9., Number 2 AbstractWebsite

Haas, Alex NogueiraGaio, Eduardo JoseWagner, Marcius ComparsiRios, Fernando SilvaCosta, Ricardo dos Santos AraujoRosing, Cassiano KuchenbeckerOppermann, Rui VicenteAlbandar, JasimSusin, CristianoengporResearch Support, Non-U.S. Gov'tBrazilRev Bras Epidemiol. 2015 Apr-Jun;18(2):515-9. doi: 10.1590/1980-5497201500020018.

Few population-based cohort studies have been established in Dentistry and this is especially true for Latin America. We conducted a population-based prospective study focusing on oral health in Porto Alegre, south Brazil, and herein we describe its methodology and discuss directions for further research. The cohort was established in 2001 using a multistage probability sample of 1,465 toothed and 121 edentulous subjects. A 5-year follow-up was performed in 2006 that included 755 individuals. The main aim of this study was to determine the pattern and risk factors for periodontal disease progression and tooth loss incidence. A full-mouth protocol was used including periodontal assessments at six sites per tooth. Primary outcomes were periodontal attachment loss and tooth loss. Oral mucosal lesions, dental plaque, gingivitis, supragingival calculus, probing depths, gingival recession, and dental caries were also assessed. This is the first population-based cohort study to focus on periodontal disease in Latin America. Findings will contribute to our understanding of the epidemiology of periodontal disease and provide valuable data for the planning and implementation of preventive and therapeutic strategies.

Gomes, SC, Abascal CC, Haas AN, Angst PD, Oppermann RV, Marcantonio RA.  2015.  Influence of supragingival biofilm control and smoking habit on Interleukin-1beta concentration. Braz Oral Res. 29:S1806-83242015000100302., Number 1 AbstractWebsite

Gomes, Sabrina CarvalhoAbascal, Carolina CoradiniHaas, Alex NogueiraAngst, Patricia Daniela MelchiorsOppermann, Rui VicenteMarcantonio, Rosemary Adriana ChiericiengComparative StudyBrazilBraz Oral Res. 2015;29(1):S1806-83242015000100302. doi: 10.1590/1807-3107BOR-2015.vol29.0115. Epub 2015 Oct 9.

This investigation compared gingival crevicular fluid (GCF) interleukin-1beta (IL-1beta) concentrations in periodontitis patients subjected to a strict supragingival biofilm control (Supra) for 6 months. Never-smokers (23) and smokers (n = 20; 19.6 +/- 11.8 cigarettes/day) moderate-to-severe chronic periodontitis patients underwent a 6 months period of supragingival control with weekly recall visits. Periodontal probing depth (PPD), bleeding on probing (BOP) and GCF samples (from different PPD category sites: 3-5 mm and 6-10 mm) were obtained at the baseline, 30, and 180 days. IL-1beta was assessed by enzyme-linked immunosorbent assay. Generalized estimating equations were used to fit prediction models of IL-1beta changes, considering the dependence between the examinations, and using only data from experimental sites. Overall IL-1beta concentrations decreased from 3.2 pg/microL to 1.9 pg/microL. Higher baseline IL-1beta concentrations were associated with higher baseline PPD values in both groups. There were no differences in IL-1beta concentrations between never-smokers and smokers over time for any PPD category. Higher baseline PPD values and the presence of BOP on day 180 were significantly associated with higher IL-1beta concentrations. A strict Supra regimen reduced IL-1beta concentrations over time in periodontitis patients. The benefits observed for smokers underline the importance of oral hygiene measures, even considering the presence of this important risk factor.

2014
Montenegro, MM, Flores MF, Colussi PR, Oppermann RV, Haas AN, Rosing CK.  2014.  Factors associated with self-reported use of mouthwashes in southern Brazil in 1996 and 2009, May. Int J Dent Hyg. 12:103-7., Number 2 AbstractWebsite

Montenegro, M MFlores, M FColussi, P R GOppermann, R VHaas, A NRosing, C KengEngland2013/09/17 06:00Int J Dent Hyg. 2014 May;12(2):103-7. doi: 10.1111/idh.12052. Epub 2013 Sep 3.

OBJECTIVE: To evaluate the association between sociodemographic factors and self-reported use of mouthwashes in a city in the south of Brazil over a time span of 13 years. METHODS: The study involves a comparison of two household surveys conducted in 1996 and 2009. Respectively, 850 and 982 households were included in each year. The mother of the family answered to a structured questionnaire to provide sociodemographic data. Self-reported use of mouthwash was evaluated in a binary manner (yes/no). Multivariate Poisson regression models were used to estimate proportion ratios (PR) and 95% confidence intervals (95% CI). RESULTS: The proportion of mouthwash use increased significantly from 10.8% to 24.2% after 13 years. The probability of using mouthwashes increased more than two times from 1996 to 2009 (PR = 2.25 95% CI 1.80-2.82). Higher probabilities for using mouthwashes were observed in households with higher income (PR = 2.63 95% CI 1.97-3.51) and in those households where the mothers had higher educational levels (PR = 1.46 95% CI 1.05-2.03). CONCLUSION: Higher family income and higher maternal educational level were associated with self-reported use of mouthwashes in a higher proportion of households.

Haas, AN, Wagner MC, Oppermann RV, Rosing CK, Albandar JM, Susin C.  2014.  Risk factors for the progression of periodontal attachment loss: a 5-year population-based study in South Brazil, Mar. J Clin Periodontol. 41:215-23., Number 3 AbstractWebsite

Haas, Alex NogueiraWagner, Marcius ComparsiOppermann, Rui VicenteRosing, Cassiano KuchenbeckerAlbandar, Jasim MSusin, CristianoengResearch Support, Non-U.S. Gov't2013/12/07 06:00J Clin Periodontol. 2014 Mar;41(3):215-23. doi: 10.1111/jcpe.12213. Epub 2014 Jan 12.

AIM: To assess sociodemographic and behavioural risk factors for periodontal attachment loss (PAL) progression after 5 years in an urban sample from south Brazil. METHODS: At baseline, 1586 subjects, 14 years and older, were derived using a multistage probabilistic sample strategy. At follow-up, 653 of 755 subjects had >/=6 teeth and were included in this analysis. A modified multiple Poisson regression was used to calculate adjusted relative risks (RR) and 95% confidence intervals (CI). RESULTS: Overall, 247 (37.8%) subjects exhibited PAL progression >/=3 mm in >/=4 teeth. Subjects older than 30 years had approximately two times higher risk of having PAL progression than younger subjects. Subjects with low education had 53% higher risk (RR = 1.53; 95% CI:1.06-2.22) of PAL progression compared to those with high education. A significant interaction between gender and smoking was observed. Among never-smokers, males were 33% more likely (RR = 1.33; 95% CI:1.06-1.66) to experience PAL progression than females. Among smokers, there was 8% increased risk of PAL progression (RR = 1.08; 95% CI:1.01-1.14) for males and 21% (RR = 1.21; 95% CI:1.11-1.33) for females per 10 packyears. Skin colour, socioeconomy, dental care and diabetes were not significantly associated with PAL progression after statistical adjustment. CONCLUSION: Sociodemographic factors and smoking are independent risk factors for PAL progression in this Brazilian population.

Susin, C, Haas AN, Albandar JM.  2014.  Epidemiology and demographics of aggressive periodontitis, Jun. Periodontol 2000. 65:27-45., Number 1 AbstractWebsite

Susin, CristianoHaas, Alex NAlbandar, Jasim MengDenmark2014/04/18 06:00Periodontol 2000. 2014 Jun;65(1):27-45. doi: 10.1111/prd.12019.

Epidemiologic studies of aggressive periodontitis have used different study designs and a range of examination methods and case definitions, and this greatly complicates the study of disease prevalence in populations. The wide range of disease case definitions, in particular, profoundly impacts the reported rate of disease, and the use of a standard disease definition is strongly recommended. Surveys of aggressive periodontitis that use only clinical examinations, without radiographic examination to confirm the presence of a distinctive pattern of tissue loss, may overestimate the prevalence of this disease, particularly when a low threshold of attachment loss is used. The prevalence of aggressive periodontitis varies significantly between populations, and differences in race/ethnicities seem to be a key factor. Studies consistently show that aggressive periodontitis is most prevalent in Africa and in populations of African descent and is least prevalent in Caucasians in Europe and North America. Among children and young adults the prevalence of this disease is higher in older than in younger age groups. Most studies show comparable disease prevalence in male and female subjects. These findings show that aggressive periodontitis is a significant health problem in certain populations. This review also highlights a lack of information on the epidemiology and demographics of this disease in many parts of the world, particularly in Asia and Africa. Epidemiologic studies of aggressive periodontitis in high-risk populations are important and could provide vital data on the determinants of this disease, and this information is needed for the establishment of effective health-promotion measures.

Costa, RS, Rios FS, Moura MS, Jardim JJ, Maltz M, Haas AN.  2014.  Prevalence and Risk Indicators of Dentin Hypersensitivity in Adult and Elderly Populations From Porto Alegre, Brazil, Feb 14. J Periodontol. AbstractWebsite

Costa, Ricardo S ARios, Fernando SMoura, Mauricio SJardim, Juliana JMaltz, MarisaHaas, Alex NENG2014/02/18 06:00J Periodontol. 2014 Feb 14.

Background: Dentin hypersensitivity (DH) is a clinical condition with potential implications for patients. However, little is known about its occurrence and determinants in the general population. The aim of this study was to assess the prevalence and risk indicators of DH in Porto Alegre, Brazil. Methods: A representative multistage probability sample of 1,023 adults aged >/=35 years was obtained. Individuals were interviewed and were clinically examined in their homes. DH was assessed dichotomously in all present teeth by a blast of air and a manual probe. Teeth restored with crowns and presenting with carious cavitation were excluded. Survey logistic regression using sampling weights was applied to assess demographics, behavioral and clinical determinants. Results: Overall prevalence estimates for DH diagnosed by air and probe were 33.4% and 34.2%. DH affected 1.0 tooth per individual, and approximately 10% of teeth with gingival recession (GR) had DH. In a multivariate model for DH diagnosed with air, females had increased chance of DH [odds ratio (OR): 2.14; 95% confidence interval (CI): 1.57-2.91]. Smoking, periodontal treatment, and GR were also associated with increased DH chances. The chance of DH was lower (OR: 0.47, 95% CI: 0.29-0.76) among individuals >/=60 than 35 to 49 years old. Oral hygiene practices, socioeconomic and educational statuses, dental visits, and gingival inflammation were not associated with DH. Conclusion: DH may be considered a concern in a Brazilian general population. Reduction of DH may be achieved by smoking cessation and periodontal health improvements.

Franzon, R, Guimaraes LF, Magalhaes CE, Haas AN, Araujo FB.  2014.  Outcomes of One-Step Incomplete and Complete Excavation in Primary Teeth: A 24-Month Randomized Controlled Trial, Apr 8. Caries Res. 48:376-383., Number 5 AbstractWebsite

Franzon, RenataGuimaraes, Lizandra FMagalhaes, Camila EHaas, Alex NAraujo, Fernando BENG2014/04/16 06:00Caries Res. 2014 Apr 8;48(5):376-383.

Aim: To compare 24-month pulp health outcomes of partial caries removal (PCR) and total caries removal (TCR) with composite restoration in primary molars. Methods: 48 children aged 3-8 years with at least one molar with a deep carious lesion were included. 120 teeth were randomized to control (TCR; n = 54; 69% class II) and test (PCR; n = 66; 63% class II) groups. Total absence of carious tissue was confirmed using a blunt-tipped probe in the TCR group. For PCR, excavation was stopped when hardened, dried dentin with a leathery consistency was achieved. Pulpotomy was performed in cases of pulp exposure. Results: Pulp exposure occurred in 2 and 27.5% of teeth treated with PCR and TCR, respectively (p < 0.01). The operative time was significantly higher for TCR than PCR. Success rates were 92 and 96% in the PCR and TCR groups, respectively (p = 0.34). The success rate tended to be lower in occlusoproximal (92%) than in occlusal (100%) lesions (p = 0.08). Conclusion: The clinical and radiographic success rates of PCR and TCR in primary teeth with deep carious lesions were high and did not differ significantly, indicating that PCR is a reliable minimally invasive approach in primary teeth and that the retention of carious dentin does not interfere with pulp vitality. Moreover, PCR provided other clinically relevant advantages over TCR, especially lower incidence of pulp exposure and lower operative time. (c) 2014 S. Karger AG, Basel.

Flores, MF, Montenegro MM, Furtado MV, Polanczyk CA, Rosing CK, Haas AN.  2014.  Periodontal status affects C-reactive protein and lipids in patients with stable heart disease from a tertiary care cardiovascular clinic, Apr. J Periodontol. 85:545-53., Number 4 AbstractWebsite

Flores, Manuela FMontenegro, Marlon MFurtado, Mariana VPolanczyk, Carisi ARosing, Cassiano KHaas, Alex Neng2013/06/29 06:00J Periodontol. 2014 Apr;85(4):545-53. doi: 10.1902/jop.2013.130255. Epub 2013 Jun 27.

BACKGROUND: There are scarce data on the impact of the periodontal condition in the control of biomarkers in patients with cardiovascular disease (CVD). The aim of this study is to assess whether periodontal inflammation and tissue breakdown are associated with C-reactive protein (CRP) and lipids in patients with stable heart disease. METHODS: This cross-sectional study included 93 patients with stable coronary artery disease (57 males; mean age: 63.5 +/- 9.8 years) who were in outpatient care for at least 6 months. After applying a structured questionnaire, periodontal examinations were performed by two calibrated periodontists in six sites per tooth at all teeth. Blood samples were collected from patients on the day of periodontal examination to determine levels of CRP, lipids, and glycated hemoglobin. Multiple linear regression models were fitted to evaluate the association among different periodontal and blood parameters controlling for sex, body mass index, glycated hemoglobin, use of oral hypoglycemic drugs, and smoking. RESULTS: Overall, the sample presented high levels of periodontal inflammation and tissue breakdown. Unadjusted mean concentrations of triglycerides (TGs), very-low-density lipoprotein cholesterol, and glucose were significantly higher in individuals with severe periodontitis. When multiple linear regression models were applied, number of teeth with clinical attachment loss >/=6 mm and presence of severe periodontitis were significantly associated with higher CRP concentrations. Bleeding on probing was significantly associated with TGs, total cholesterol, and non-high-density lipoprotein cholesterol. CONCLUSION: In this sample of patients with stable CVD, current periodontal inflammation and tissue breakdown are associated with cardiovascular inflammatory markers, such as CRP and lipid profile.

2013
Bressani, AE, Mariath AA, Haas AN, Garcia-Godoy F, de Araujo FB.  2013.  Incomplete caries removal and indirect pulp capping in primary molars: a randomized controlled trial, Aug. Am J Dent. 26:196-200., Number 4 AbstractWebsite

Bressani, Ana Eliza LemesMariath, Adriela Azevedo SouzaHaas, Alex NogueiraGarcia-Godoy, Franklinde Araujo, Fernando BorbaengComparative StudyRandomized Controlled Trial2014/04/04 06:00Am J Dent. 2013 Aug;26(4):196-200.

PURPOSE: To compare the effect of incomplete caries removal (ICR) and indirect pulp capping (IPC) with calcium hydroxide (CH) or an inert material (wax) on color, consistency and contamination of the remaining dentin of primary molars. METHODS: This double-blind, parallel-design, randomized controlled trial included 30 children presenting one primary molar with deep caries lesion. Children were randomly assigned after ICR to receive IPC with CH or wax. All teeth were then restored with resin composite. Baseline dentin color and consistency were evaluated after ICR, and dentin samples were collected for contamination analyses using scanning electron microscopy. After 3 months, restorations were removed and the three parameters were re-evaluated. In both groups, dentin became significantly darker after 3 months. RESULTS: No cases of yellow dentin were observed after 3 months with CH compared to 33.3% of the wax cases (P < 0.05). A statistically significant difference over time was observed only for CH regarding consistency. CH stimulated a dentin hardening process in a statistically higher number of cases than wax (86.7% vs. 33.3%; P = 0.008). Contamination changed significantly over time in CH and wax without significant difference between groups. It was concluded that CH and wax arrested the carious process of the remaining carious dentin after indirect pulp capping, but CH showed superior dentin color and consistency after 3 months.

Greggianin, BF, Oliveira SC, Haas AN, Oppermann RV.  2013.  The incidence of gingival fissures associated with toothbrushing: crossover 28-day randomized trial, Apr. J Clin Periodontol. 40:319-26., Number 4 AbstractWebsite

Greggianin, Bruna FrizonOliveira, Sara CioccariHaas, Alex NogueiraOppermann, Rui VicenteengRandomized Controlled TrialResearch Support, Non-U.S. Gov't2013/02/22 06:00J Clin Periodontol. 2013 Apr;40(4):319-26. doi: 10.1111/jcpe.12072. Epub 2013 Feb 21.

AIM: To compare the incidence of gingival fissures after the use of soft and medium-hard toothbrushes. MATERIAL & METHODS: Overall, 35 participants (14-20 years old), with periodontal attachment loss (PAL)

Saffi, MA, Furtado MV, Montenegro MM, Ribeiro IW, Kampits C, Rabelo-Silva ER, Polanczyk CA, Rosing CK, Haas AN.  2013.  The effect of periodontal therapy on C-reactive protein, endothelial function, lipids and proinflammatory biomarkers in patients with stable coronary artery disease: study protocol for a randomized controlled trial. Trials. 14:283. AbstractWebsite

Saffi, Marco Aurelio LumertzFurtado, Mariana VargasMontenegro, Marlon MunhozRibeiro, Ingrid Webb JosephsonKampits, CassioRabelo-Silva, Eneida RejanePolanczyk, Carisi AnneRosing, Cassiano KuchenbeckerHaas, Alex NogueiraengResearch Support, Non-U.S. Gov'tEngland2013/09/10 06:00Trials. 2013 Sep 6;14:283. doi: 10.1186/1745-6215-14-283.

BACKGROUND: Scarce information exists regarding the preventive effect of periodontal treatment in the recurrence of cardiovascular events. Prevention may be achieved by targeting risk factors for recurrent coronary artery disease (CAD) in patients with previous history of cardiovascular events. The aim of this trial is to compare the effect of two periodontal treatment approaches on levels of C-reactive protein, lipids, flow-mediated dilation and serum concentrations of proinflammatory and endothelial markers in stable CAD patients with periodontitis over a period of 12 months. METHODS/DESIGN: This is a randomized, parallel design, examiner blinded, controlled clinical trial. Individuals from both genders, 35 years of age and older, with concomitant diagnosis of CAD and periodontitis will be included. CAD will be defined as the occurrence of at least one of the following events 6 months prior to entering the trial: documented history of myocardial infarction; surgical or percutaneous myocardial revascularization and lesion >50% in at least one coronary artery assessed by angiography; presence of angina and positive noninvasive testing of ischemia. Diagnosis of periodontitis will be defined using the CDC-AAP case definition (>/=2 interproximal sites with clinical attachment loss >/=6 mm and >/=1 interproximal site with probing depth >/=5 mm). Individuals will have to present at least ten teeth present to be included. One hundred individuals will be allocated to test (intensive periodontal treatment comprised by scaling and root planing) or control (community periodontal treatment consisting of one session of supragingival plaque removal only) treatment groups. Full-mouth six sites per tooth periodontal examinations and subgingival biofilm samples will be conducted at baseline, 3, 6 and 12 months after treatment. The primary outcome of this study will be C-reactive protein changes over time. Secondary outcomes include levels of total cholesterol, LDL-C, HDL-C, triglycerides, IL-1beta, IL-6, TNFalpha, fibrinogen, ICAM-1, VCAM-1 and E-selectin. These outcomes will be assessed at all time points over 12 months. Flow-mediated dilation will be assessed at baseline, 1, 3 and 6 months after periodontal therapy. DISCUSSION: This trial will provide new evidence regarding the effect of periodontal treatment on risk markers for recurrence of cardiovascular events in stable coronary artery disease patients. TRIAL REGISTRATION NUMBER: ClinicalTrials.gov Identifier, NCT01609725.

2012
Gaio, EJ, Haas AN, Carrard VC, Oppermann RV, Albandar J, Susin C.  2012.  Oral health status in elders from South Brazil: a population-based study, Sep. Gerodontology. 29:214-23., Number 3 AbstractWebsite

Gaio, Eduardo JHaas, Alex NCarrard, Vinicius COppermann, Rui VAlbandar, JasimSusin, CristianoengResearch Support, Non-U.S. Gov'tEngland2012/04/11 06:00Gerodontology. 2012 Sep;29(3):214-23. doi: 10.1111/j.1741-2358.2011.00617.x. Epub 2012 Apr 9.

OBJECTIVE: To assess the oral health status of community-dwelling adults aged 60 years and older from southern Brazil and to determine demographics, socioeconomic, behavioural and dental risk indicators. MATERIALS AND METHODS: This cross-sectional study used a multistage, probability sampling method to draw a representative sample of the metropolitan area of Porto Alegre, Brazil. A subsample of 217 subjects was included in this analysis. Oral mucosal lesions, dental caries, tooth loss and periodontal status (full-mouth, six sites per tooth exam) were assessed by calibrated examiners. RESULTS: Prevalence of edentulism was 39.5%, and mean tooth loss was 20.2 (SE = 0.6). Older individuals [Odds Ratio (OR) = 2.2], women (OR = 2.3), white people (OR = 5.9), individuals of lower socioeconomic status (OR = 5.6) and smokers (OR = 3.5) had higher likelihood of being edentulous. Approximately 36% of dentate individuals had caries and/or restoration affecting, in average, 5.0 teeth. Periodontitis affected 79% of subjects, and it was associated with older age (OR = 4.0), men (OR = 3.4) and large amounts of supragingival plaque (OR = 3.0). CONCLUSION: Poor oral health was observed in this elderly population from South Brazil. Sociodemographic disparities accounted for most of the burden of disease and treatment needs.

Mendez, M, Carrard VC, Haas AN, Lauxen Ida S, Barbachan JJ, Rados PV, Sant'Ana Filho M.  2012.  A 10-year study of specimens submitted to oral pathology laboratory analysis: lesion occurrence and demographic features, May-Jun. Braz Oral Res. 26:235-41., Number 3 AbstractWebsite

Mendez, MarinaCarrard, Vinicius CoelhoHaas, Alex NogueiraLauxen, Isabel da SilvaBarbachan, Joao Jorge DinizRados, Pantelis VarvakiSant'Ana Filho, ManoelengResearch Support, Non-U.S. Gov'tBrazil2012/05/30 06:00Braz Oral Res. 2012 May-Jun;26(3):235-41.

The purpose of the present paper was to describe the range of lesions histologically diagnosed in an oral pathology laboratory in southern Brazil. A retrospective study of 8,168 specimen analyses recorded between 1995 and 2004 was conducted. The records were retrieved from the Oral Pathology Laboratory, School of Dentistry, Federal University of Rio Grande do Sul, RS, Brazil. A total of 6,831 valid cases (83.63%) were examined. Of these, inflammatory lesions were the most common occurrences (n = 4,320; 63.24%). Benign and malignant tumors accounted for 7.66% (n = 523) and 1.9% (n = 130) of the occurrences, respectively. Significant associations were observed between nonneoplastic proliferative disorders and benign mesenchymal tumors in females, and between squamous cell carcinoma and leukoplakia in males. Most diagnoses were benign in nature and had an inflammatory etiology. The association of some demographic characteristics with the occurrence of lesions suggests that these characteristics should be considered in performing differential diagnoses.

Haas, AN, Silva-Boghossian CM, Colombo AP, Susin C, Albandar JM, Oppermann RV, Rosing CK.  2012.  Adjunctive azithromycin in the treatment of aggressive periodontitis: microbiological findings of a 12-month randomized clinical trial, Jul. J Dent. 40:556-63., Number 7 AbstractWebsite

Haas, Alex NogueiraSilva-Boghossian, Carina MacielColombo, Ana PaulaSusin, CristianoAlbandar, Jasim MOppermann, Rui VicenteRosing, Cassiano KuchenbeckerengComparative StudyRandomized Controlled TrialResearch Support, Non-U.S. Gov'tEngland2012/03/27 06:00J Dent. 2012 Jul;40(7):556-63. doi: 10.1016/j.jdent.2012.03.004. Epub 2012 Mar 20.

OBJECTIVES: To compare the subgingival microbiological outcomes of azithromycin or placebo as adjuncts to scaling and root planing (SRP) in the treatment of aggressive periodontitis (AgP), and to secondarily evaluate the microbiological effect of supragingival scaling in AgP patients. METHODS: Twenty-four AgP subjects 13-26 years of age received a 15-day programme of supragingival scaling (SC) and were then randomly assigned to SRP with systemic azithromycin or placebo. Subgingival samples were taken with sterile paper points at baseline, 15 days after SC, and at 3, 6 and 12 months following SRP. Microbiological analysis was performed by the checkerboard DNA-DNA hybridization. RESULTS: Changes in bacterial levels from baseline to 15 days after SC were similar in the 2 groups. When subjects were analysed as a single group, significant reductions after SC were observed for Actinomyces gerencseriae, Capnocytophaga ochracea, and Treponema denticola. During the 12-month follow-up, levels of most of the bacteria decreased in both groups in a similar pattern. For instance, Actinomyces israelli, Veillonella parvula, Streptococcus gordonii, C. ochracea, Eikenella corrodens, Eubacterium nodatum, Fusobacterium periodonticum and Fusobacterium nucleatum ssp. polymorphum decreased significantly within the groups. CONCLUSIONS: Azithromycin was ineffective in lowering the subgingival levels of important putative periodontal pathogens in young AgP subjects compared to placebo. CLINICAL SIGNIFICANCE: Scaling and root planing with adjunctive systemic azithromycin provides little additional benefit compared to placebo in reductions of major subgingival periodontal pathogens.

Haas, AN, Gaio EJ, Oppermann RV, Rosing CK, Albandar JM, Susin C.  2012.  Pattern and rate of progression of periodontal attachment loss in an urban population of South Brazil: a 5-years population-based prospective study, Jan. J Clin Periodontol. 39:1-9., Number 1 AbstractWebsite

Haas, Alex NogueiraGaio, Eduardo JoseOppermann, Rui VicenteRosing, Cassiano KuchenbeckerAlbandar, Jasim MSusin, CristianoengDenmark2011/11/19 06:00J Clin Periodontol. 2012 Jan;39(1):1-9. doi: 10.1111/j.1600-051X.2011.01818.x. Epub 2011 Nov 14.

AIM: The aim of this 5-years longitudinal study was to investigate the pattern and rate of periodontal attachment loss (PAL) progression in an urban population in South Brazil. METHODS: In 2001, a multistage probability sampling strategy was used to derive a representative sample of 1,465 dentate individuals from Porto Alegre, Brazil. Five years later, 697 dentate individuals (294M/403F, mean age: 37.9 +/- 13.3) were available for follow-up. PAL was assessed by calibrated examiners using a full-mouth protocol. Estimates of proximal PAL progression and standard errors (SE) are reported. RESULTS: Fifty-six per cent (SE: 1.9) and 36% (SE: 1.8) of subjects showed PAL progression >/=3 mm affecting >/=2 and >/=4 teeth respectively. PAL progression >/=3 mm was mostly localized affecting 3.8 (SE: 0.2) teeth and 5.7 (SE: 0.3) sites. Annual PAL progression was, on average, 0.3 mm (SE: 0.01). Significant differences in PAL progression were observed according to age, gender, race and socioeconomic status. PAL progression increased with age reaching the highest progression rate in the 40-49 years cohort, and then decreased in older age groups. PAL progression was consistently higher among males and non-Whites than females and whites. CONCLUSION: A large proportion of this urban Brazilian sample was affected by PAL progression underscoring the need for health promotion initiatives aiming at preventing progression of destructive periodontal disease.

Haas, AN, Seleme F, Segatto P, Susin C, Albandar J, Oppermann RV, Fontanella VR, Rosing CK.  2012.  Azithromycin as an adjunctive treatment of aggressive periodontitis: radiographic findings of a 12-month randomized clinical trial, Aug. Am J Dent. 25:215-9., Number 4 AbstractWebsite

Haas, Alex NogueiraSeleme, FrancineSegatto, PaulaSusin, CristianoAlbandar, JasimOppermann, Rui VicenteFontanella, Vania ReginaRosing, Cassiano KuchenbeckerengComparative StudyRandomized Controlled TrialResearch Support, Non-U.S. Gov't2012/10/23 06:00Am J Dent. 2012 Aug;25(4):215-9.

PURPOSE: To compare the 12-month radiographic outcomes following the use of azithromycin or placebo as adjuncts to non-surgical periodontal treatment of AgP. METHODS: 17 aggressive periodontitis (AgP) subjects 13-26 years old were randomly assigned to receive scaling and root planing (SRP) with systemic azithromycin or placebo. Standardized radiographs were taken at baseline and 12 months postoperatively. Recall visits consisting of oral prophylaxis and oral hygiene instructions were performed during the 12 months. Digital image subtraction analysis and linear bone measurements were conducted by a blinded and calibrated examiner. Student t-tests were used for within and between-groups comparisons. ANCOVA was applied for between-group comparisons of changes in linear bone level adjusting for baseline values. RESULTS: There were significant gains in linear bone levels in the azithromycin (0.55 +/- 0.10 mm) and placebo (0.42 +/- 0.07 mm) groups between the baseline and 12-month postoperative visits. There were also significant gains in bone density in the two treatment groups. No significant differences were observed between the two treatments in the amount of linear bone gain or bone density during the follow-up period. The use of azithromycin as an adjunct to SRP in the treatment of AgP did not result in significant radiographic bone level changes compared to placebo.

Colussi, PR, Haas AN, Oppermann RV, Rosing CK.  2012.  Factors associated with changes in self-reported dentifrice consumption in a Brazilian group from 1996 and 2009. Braz Dent J. 23:737-45., Number 6 AbstractWebsite

Colussi, Paulo Roberto GrafittiHaas, Alex NogueiraOppermann, Rui VicenteRosing, Cassiano KuchenbeckerengBrazil2013/01/23 06:00Braz Dent J. 2012;23(6):737-45.

The aim of the study was to determine factors associated with changes in self-reported dentifrice consumption in an urban population group over 13 years. This study evaluated two surveys of 671 and 688 households sampled in the urban area of a city from Southern Brazil in 1996 and 2009, respectively. The mother of the family was asked to answer a structured questionnaire about demographics, socioeconomic and behavioral variables. The primary outcome was obtained by questioning "how long does a dentifrice tube last in your house?" The cut-off point of duration was less than 1 month. It was used to determine high consumption of dentifrice (HCD). Associations between HCD and independent variables were evaluated by multivariable Poisson regression. There was a significant decrease of 20% (81.2% to 61.2%) in the prevalence of HCD between 1996 and 2009, resulting in a crude annual decrease of 1.54%. Mother's age, family income, dental assistance, mother's brushing frequency and number of household members that use a toothbrush were significantly associated with HCD independent from the year of survey. The prevalence ratio (PR) of HCD for the year of survey was 0.75, indicating an overall decrease of 25% in the probability of HCD from 1996 to 2009. Probabilities of HCD also decreased over the 13 years among the strata of education, number of household members and reason for choice of dentifrice. It may be concluded that the factors associated with the observed decrease were higher educational levels, larger number of household members and reasons for choosing a dentifrice related to preventive/therapeutic effects.

2011
Colussi, PR, Haas AN, Oppermann RV, Rosing CK.  2011.  [Consumption of toothpaste and associated factors in a Brazilian population group], Mar. Cad Saude Publica. 27:546-54., Number 3 AbstractWebsite

Colussi, Paulo Roberto GrafittiHaas, Alex NogueiraOppermann, Rui VicenteRosing, Cassiano KuchenbeckerporEnglish AbstractBrazil2011/04/27 06:00Cad Saude Publica. 2011 Mar;27(3):546-54.

This cross-sectional study evaluated the consumption of fluoridated dentifrice and associated factors in Passo Fundo, Rio Grande do Sul State, Brazil. A total of 688 households were selected. A structured questionnaire was answered by the mother to obtain demographics, habits, and toothpaste consumption. Household toothpaste consumption was considered low when a tube lasted > 1 month and high when < 1 month. Logistic regression models were applied to evaluate the association between consumption and independent variables. 61.2% of households showed high toothpaste consumption. In the multivariate model, mother's age > 50 years (OR = 1.62; 95%CI: 1.02-2.61), mother's brushing frequency (OR = 2.53; 95%CI: 1.53-4.16), number of people brushing (OR = 5.69; 95%CI: 3.68-8.81), and cosmetic features in choice of the dentifrice (OR = 1.64; 95%CI: 1.03-2.61) showed the highest odds of high toothpaste consumption.

Susin, C, Haas AN, Valle PM, Oppermann RV, Albandar JM.  2011.  Prevalence and risk indicators for chronic periodontitis in adolescents and young adults in south Brazil, Apr. J Clin Periodontol. 38:326-33., Number 4 AbstractWebsite

Susin, CristianoHaas, Alex NValle, Patricia MOppermann, Rui VAlbandar, Jasim MengResearch Support, Non-U.S. Gov'tDenmark2011/02/09 06:00J Clin Periodontol. 2011 Apr;38(4):326-33. doi: 10.1111/j.1600-051X.2011.01699.x. Epub 2011 Feb 7.

AIM: To describe the distribution of clinical attachment loss (CAL) and to study risk indicators for chronic periodontitis in a large population-based sample of adolescents and young adults from south Brazil. MATERIAL AND METHODS: This cross-sectional study used a subset of data from a larger survey representative of Porto Alegre, Brazil. The sample consisted of 612 individuals (291 males/321 females) aged 14-29 years. Full-mouth, six sites per tooth clinical examinations were performed by calibrated periodontists. Chronic periodontitis was defined as CAL 3mm affecting two or more teeth. Aggressive periodontitis cases were excluded from the analysis. RESULTS: CAL 3 and 5mm affected 50.4% and 17.4% of subjects and 9.7% and 1.1% of teeth, respectively. Prevalence of chronic periodontitis ranged between 18.2% and 72.0% among subjects 14-19 and 24-29 years old, respectively. In the multivariable logistic regression analysis, older age [odds ratio (OR)=2.6, 95% confidence interval (CI)=1.7-3.9 and OR=7.2, 95% CI=3.7-14.0 for 20-24 and 25-29 years old, respectively], low socioeconomic status (OR=1.9, 95% CI=1.4-2.7), heavy smoking (OR=1.7, 95% CI=1.1-2.7) and larger amounts of calculus (OR=2.0, 95% CI=1.2-3.2) were significantly associated with chronic periodontitis. CONCLUSION: This population of adolescents and young adults had a high prevalence of chronic periodontitis, and its presence was associated with age, socioeconomic status, smoking and calculus.

2010
Oppermann, RV, Haas AN, Villoria GE, Primo LG, Serra-Negra JM, Ferreira EF, Pannuti CM.  2010.  Proposal for the teaching of the chemical control of supragingival biofilm. Braz Oral Res. 24 Suppl 1:33-6. AbstractWebsite

Oppermann, Rui VicenteHaas, Alex NogueiraVilloria, German Eduardo MiguelPrimo, Laura GuimaraesSerra-Negra, Junia MFerreira, Efigenia Ferreira ePannuti, Claudio MendesengBrazil2010/10/05 06:00Braz Oral Res. 2010;24 Suppl 1:33-6.

The mechanical control of supragingival biofilm is accepted as one of the most important measures to treat and prevent dental caries and periodontal diseases. Nevertheless, maintaining dental surfaces biofilm-free is not an easy task. In this regard, chemical agents, mainly in the form of mouthwashes, have been studied to help overcome the difficulties involved in the mechanical control of biofilm. The aim of this paper was to discuss proposals for the teaching of supragingival chemical control (SCC) in order to improve dentists' knowledge regarding this clinical issue. Firstly, the literature regarding the efficacy of antiseptics is presented, clearly showing that chemical agents are clinically effective in the reduction of biofilm and gingival inflammation when used as adjuvant agents to mechanical control. Thus, it is suggested that the content related to SCC be included in the curricular grid of dental schools. Secondly, some essential topics are recommended to be included in the teaching of SCC as follows: skills and competencies expected of a graduate dentist regarding SCC; how to include this content in the curricular grid; teaching-learning tools and techniques to be employed; and program content.

2009
Haas, AN, Rosing CK, Oppermann RV, Albandar JM, Susin C.  2009.  Association among menopause, hormone replacement therapy, and periodontal attachment loss in southern Brazilian women, Sep. J Periodontol. 80:1380-7., Number 9 AbstractWebsite

Haas, Alex NRosing, Cassiano KOppermann, Rui VAlbandar, Jasim MSusin, CristianoengComparative StudyResearch Support, Non-U.S. Gov't2009/09/03 06:00J Periodontol. 2009 Sep;80(9):1380-7. doi: 10.1902/jop.2009.090082.

BACKGROUND: The aim of the present study was to evaluate the association among menopause, hormone replacement therapy (HRT), and periodontal attachment loss (PAL). METHODS: Three hundred twenty-eight women (40 to 69 years old) who participated in a previous oral survey in South Brazil were included. Women who reported not having their monthly menstrual cycles for >or=12 months were classified as postmenopausal. Postmenopausal women were categorized according to HRT. Women with >or=30% teeth with PAL >or=5 mm were classified as having periodontitis. The data were analyzed using a multivariable logistic regression model and adjusted for age, smoking, socioeconomic status, and dental care. RESULTS: The prevalence of periodontitis was significantly greater among postmenopausal women not using HRT (HRT-) than among premenopausal women (64.4% versus 46.3%; P = 0.005). Similarly, the multivariable analysis demonstrated a significantly higher chance of having periodontitis in postmenopausal HRT- women than in premenopausal women (odds ratio [OR], 2.1; 95% confidence interval [CI]: 1.1 to 4.0). In contrast, no significant differences were observed in the prevalence of periodontitis between postmenopausal women using HRT (HRT+) and premenopausal women (48.8% versus 46.3%; P = 0.77). After adjusting for cofactors, postmenopausal HRT+ women did not have a greater likelihood of having periodontitis than premenopausal women (OR, 1.2; 95% CI: 0.6 to 2.5). No significant differences in tooth loss were observed among the study groups after adjusting for confounders. CONCLUSIONS: Postmenopausal HRT- women had a greater chance of having periodontitis than premenopausal women. In contrast, postmenopausal HRT+ women and premenopausal women had similar periodontal status. HRT may have a beneficial effect on periodontal health.