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2009
Mariath, AA, Haas AN, Fischer CM, de Araujo FB, Rosing CK.  2009.  Professional toothbrushing as a method for diagnosing gingivitis in 3- to 6-year-old preschool children. Oral Health Prev Dent. 7:315-21., Number 4 AbstractWebsite

Mariath, Adriela Azevedo SouzaHaas, Alex NogueiraFischer, Claudia Martinewskide Araujo, Fernando BorbaRosing, Cassiano KuchenbeckerengComparative StudyRandomized Controlled TrialValidation StudiesEngland2009/12/17 06:00Oral Health Prev Dent. 2009;7(4):315-21.

PURPOSE: The aim of the present crossover study was to evaluate professional toothbrushing as a method for diagnosing gingivitis in children. MATERIALS AND METHODS: Thirty-four preschool children who had gingival bleeding > or = 10% and without proximal restorations/carious lesions were included in the study. Examinations comprised two gingival indices recorded at a 15-min interval, in the following sequences: the Ainamo and Bay gingival bleeding index (GBI1) followed by the brushing index (BI2) and vice versa (BI1-GBI2). Half of the children started the study in the first sequence and the other half in the second. After a 3- to 4-day washout period, the indices were again recorded with individuals changing the sequences. Data analysis considered GBI as the gold standard, and sensitivity (SE), specificity (SP), positive predictive value (PPV) and negative predictive value (NPV) were also calculated. RESULTS: The overall mean value of gingival bleeding at the first examination was 18.85 +/- 9.24%. Validation of toothbrushing performed after GBI (BI2) resulted in values for SE, SP, PPV and NPV of 0.59 (95% CI, 0.55 to 0.63), 0.92 (95% CI, 0.91 to 0.93), 0.64 (95% CI, 0.60 to 0.69) and 0.90 (95% CI, 0.89 to 0.92), respectively. BI performed before GBI (BI1) resulted in similar SE, SP, PPV and NPV. Percentage agreement of GBI1-BI2 and BI1-GBI2 was 83.5% and 85.9%, respectively. CONCLUSIONS: Professional toothbrushing can be suggested as a method for the diagnosis of gingival inflammatory status in children, especially as an indicator of gingival health.

2008
Mariath, AA, Bressani AE, Haas AN, Araujo FB, Rosing CK.  2008.  Professional flossing as a diagnostic method for gingivitis in the primary dentition, Oct-Dec. Braz Oral Res. 22:316-21., Number 4 AbstractWebsite

Mariath, Adriela Azevedo SouzaBressani, Ana Eliza LemesHaas, Alex NogueiraAraujo, Fernando Borba deRosing, Cassiano KuchenbeckerengValidation StudiesBrazil2009/01/17 09:00Braz Oral Res. 2008 Oct-Dec;22(4):316-21.

The aim of this study was to evaluate flossing as a diagnostic method for interproximal gingival bleeding in children. For this crossover study, 23 pre-schoolchildren presenting neither restorations nor approximal carious cavities and with at least 15% of gingival bleeding sites were selected. Examinations were performed at three different moments (3-4 days interval). Examinations comprised repeated measurements of two gingival indices with a 10-minute interval in the following sequences: the Ainamo & Bay Gingival Bleeding Index (GBI) followed by the Carter & Barnes flossing index (CBI); CBI followed by GBI; and GBI followed by GBI. Data analysis was performed only for the interproximal sites, considering the GBI as the gold-standard. Agreement between indices, sensitivity (SE), specificity (SP), positive (PPV) and negative predictive values (NPV) were estimated. Percentage agreements in sequences GBI-CBI, CBI-GBI and GBI-GBI were 70.3%, 76.4% and 84.5%, respectively. Validation of flossing in the first sequence (GBI-CBI) resulted in values of 0.61 (95%CI 0.53 - 0.68), 0.72 (95%CI 0.69 - 0.76), 0.33 (95%CI 0.28 - 0.39) and 0.89 (95%CI 0.86 - 0.92) respectively for SE, SP, PPV and NPV. It can be concluded that professional flossing is a useful tool in the diagnosis of interproximal gingival inflammatory status in children, especially in conditions of gingival health.

Haas, AN, de Castro GD, Moreno T, Susin C, Albandar JM, Oppermann RV, Rosing CK.  2008.  Azithromycin as an adjunctive treatment of aggressive periodontitis: 12-months randomized clinical trial, Aug. J Clin Periodontol. 35:696-704., Number 8 AbstractWebsite

Haas, Alex Nde Castro, Gabriel DMoreno, TatianaSusin, CristianoAlbandar, Jasim MOppermann, Rui VRosing, Cassiano KengComparative StudyRandomized Controlled TrialResearch Support, Non-U.S. Gov'tDenmark2008/07/18 09:00J Clin Periodontol. 2008 Aug;35(8):696-704. doi: 10.1111/j.1600-051X.2008.01254.x. Epub 2008 Jul 9.

AIM: To assess the effect of systemic azithromycin as a supplement to scaling and root planing (SRP) in the treatment of aggressive periodontitis (AgP). MATERIAL AND METHODS: Twenty-four individuals (13-26 years old) underwent a plaque control program, and then were treated with SRP. Subjects were assigned randomly into two groups; the test group used 500 mg azithromycin once a day for 3 days, whereas the control group used a placebo. Clinical variables were assessed at baseline, 3, 6, 9, and 12 months. The periodontal status at baseline and 12 months was compared using the Wald test, and adjusting for the effect of clustering of teeth within subjects. RESULTS: There were no significant differences in visible plaque, gingival bleeding, and supragingival calculus between groups throughout the study. Periodontal probing depth (PPD) and clinical attachment level improved significantly from baseline to 12 months in both groups, with the test group showing significantly more reduction in mean PPD compared with controls (2.88 mm versus 1.85 mm, respectively, p=0.025). Subjects administering azithromycin showed a higher percentage of teeth with attachment gain >or=1 mm (81.34 versus 63.63, p=0.037), whereas the controls had higher percentage of teeth with attachment loss >or=1 mm (11.57 versus 2.24, p=0.015). CONCLUSIONS: The adjunctive use of azithromycin has the potential to improve periodontal health of young patients with AgP.

2007
Haas, AN, Silveira EM, Rosing CK.  2007.  Effect of tongue cleansing on morning oral malodour in periodontally healthy individuals. Oral Health Prev Dent. 5:89-94., Number 2 AbstractWebsite

Haas, Alex NogueiraSilveira, Elcia Maria VarizeRosing, Cassiano KuchenbeckerengRandomized Controlled TrialEngland2007/08/29 09:00Oral Health Prev Dent. 2007;5(2):89-94.

PURPOSE: The aim of this randomised single-blind, cross-over trial was to assess the effect of tongue cleansing on morning oral malodour in periodontally healthy subjects. MATERIALS AND METHODS: Ten systemically healthy non-smoker subjects (6 males, 4 females), 24-38 years of age, completed two 4-day periods of oral hygiene cessation with a 7-day wash-out period. In one of these test periods, subjects were instructed to clean their tongues with a tongue scraper 2-3 times a day. Participants presented at least 20 teeth, without cavities, overhanging restorations/prostheses or periodontitis, and had no history of previous periodontal therapy or use of antibiotics in the 3 months prior to the study. Volatile sulphur compounds (VSC; Interscan Halimeter) and organoleptic scores were measured in exhaled mouth air once a day, early in the morning, by one examiner. Comparisons were performed using Wilcoxon's signed rank test and Friedman's test (alpha = 0.05). RESULTS: VSC levels at baseline were 206.3 ppb (SD 139.8) and 191.4 ppb (SD 127.7) for periods of usage and non-usage of the scraper respectively (p > 0.05). VSC levels did not change significantly during the 4 days, independent of tongue cleansing (Friedman, p > 0.05). Only at day 3 did the use of the tongue scraper lead to a significantly lower level of VSC compared with controls (131.1 ppb and 199.3 ppb respectively). No significant differences in organoleptic scores were observed between groups at baseline. During the whole experimental period, there were also no significant changes in organoleptic scores when individuals used or did not use the tongue scraper. CONCLUSION: Tongue cleansing with a scraper was unable to prevent morning oral malodour in the absence of tooth cleaning in periodontally healthy individuals.

2006
Susin, C, Haas AN, Opermann RV, Albandar JM.  2006.  Tooth loss in a young population from south Brazil, Spring. J Public Health Dent. 66:110-5., Number 2 AbstractWebsite

Susin, CristianoHaas, Alex NOpermann, Rui VAlbandar, Jasim MengResearch Support, Non-U.S. Gov't2006/05/23 09:00J Public Health Dent. 2006 Spring;66(2):110-5.

OBJECTIVES: To assess the prevalence, extent, and risk indicators of tooth loss in a representative young urban population from south Brazil. METHODS: A representative sample was drawn using a multi-stage probability cluster sampling strategy, and consisted of 612 subjects 14-29 years of age in the metropolitan area of Porto Alegre, Brazil. A clinical examination was carried out by 4 calibrated examiners in a mobile examination center. RESULTS: The prevalence of tooth loss was 44.8%, 26%, and 60%, and the mean tooth loss was 1.4, 0.6, and 2.4 teeth in the age groups 14-29, 14-19 and 25-29 years, respectively. First molars were the most frequently missing teeth, and the mandibular incisors and canines were the least missing teeth. Tooth loss increased sharply with age, and was similar in males and females. Having > or =4 missing teeth was significantly associated with low socioeconomic status and heavy smoking, and was significantly more likely in persons who had > or =2 teeth with caries/fillings and/or > or =5 mm attachment loss. CONCLUSION: Tooth loss is a dental health concern in this young Brazilian population. Community-based oral diseases prevention programs targeting groups having these risk factors should be implemented to reduce tooth loss.

Castro, GD, Oppermann RV, Haas AN, Winter R, Alchieri JC.  2006.  Association between psychosocial factors and periodontitis: a case-control study, Feb. J Clin Periodontol. 33:109-14., Number 2 AbstractWebsite

Castro, G D COppermann, R VHaas, A NWinter, RAlchieri, J CengComparative StudyDenmark2006/01/31 09:00J Clin Periodontol. 2006 Feb;33(2):109-14.

OBJECTIVE: This case-control study investigated the association between life events, anxiety, and depression with periodontitis. METHODOLOGY: The study counted with 165 individuals, both sexes, 35-60 years of age. Case group included 96 individuals suffering from periodontal disease; and in control group 69 subjects with no history of periodontitis. Clinical examinations were performed by a single examiner. Psychological assessment included four inventories: Life Events Scale, Beck Anxiety Inventory, State-Trait Anxiety Inventory and Beck Depression Inventory. Bivariate and multivariate logistic regression analyses were performed to compare cases and controls. RESULTS: Mean probing depth and clinical attachment level were 3.44+/-0.80 and 4.01+/-1.61 in the case group and 1.96+/-0.19 and 0.95+/-0.50 in the control group, respectively (p<0.05). Positive association of periodontitis with age (odds ratio (OR)=1.15 95% confidence interval (CI): 1.06-1.24), male gender (OR=2.71, CI: 1.13-6.49), smoking (OR=6.05, CI: 1.67-21.94) and educational level (OR=6.49, CI: 1.14-36.95) was confirmed. Bivariate analysis did not demonstrate significant mean differences in life events, anxiety symptoms, trait or state of anxiety, or depression symptoms between cases and controls. Multivariate logistic regression, controlling for confounding factors, demonstrated no significant association between psychosocial factors and periodontal disease. CONCLUSIONS: Within the limits of this study it is possible to conclude that there was no significant association between periodontitis and the psychosocial factors analysed.

2004
Susin, C, Haas AN, Oppermann RV, Haugejorden O, Albandar JM.  2004.  Gingival recession: epidemiology and risk indicators in a representative urban Brazilian population, Oct. J Periodontol. 75:1377-86., Number 10 AbstractWebsite

Susin, CristianoHaas, Alex NOppermann, Rui VHaugejorden, OlaAlbandar, Jasim MengResearch Support, Non-U.S. Gov't2004/11/26 09:00J Periodontol. 2004 Oct;75(10):1377-86.

BACKGROUND: Gingival recession is a common manifestation of periodontal disease, but it is also associated with other risk factors. A few studies have investigated the epidemiology and risk factors of this condition. This study describes the epidemiology of gingival recession in a representative, urban Brazilian population and assesses various risk indicators. METHODS: A representative sample of 1,460 subjects was selected using a multi-stage, probability, cluster sampling strategy. The subjects were interviewed using a structured questionnaire and had a full-mouth clinical examination in a mobile examination center. RESULTS: More than half (51.6%) and 22.0% of the individuals and 17.0% and 5.8% of teeth per individual showed gingival recession > or = 3 mm and > or = 5 mm, respectively. The prevalence, extent, and severity of recession correlated with age. Recession showed a nonlinear relationship with age, with 25 to 50 year olds showing the highest level of recession. Males aged > or = 30 years showed significantly higher prevalence and extent of gingival recession than females. The percentage of teeth with recession was significantly higher in the lower socioeconomic groups irrespective of age, and in subjects > or = 30 years of age with irregular dental care than in subjects with regular care. Using a multivariable model, cigarette smoking and presence of supragingival calculus were the factors most significantly associated with localized and generalized recession, whereas gender, dental visits, and socioeconomic status were not significant risk indicators. CONCLUSIONS: The high level of gingival recession in this Brazilian population may be primarily related to destructive periodontal disease and is significantly associated with a high level of supragingival dental calculus and cigarette smoking. Population-based programs aimed at the prevention of periodontal diseases may reduce the prevalence of severe gingival recession in this and similar populations.