FERNANDO ERTHAL

The degree to which a group of fossils reflects the original community from which it was derived can be estimated by comparing living communities with locally accumulated dead remains. Such live–dead approaches (LA/DA) can provide important baseline information on the ecological structure of ancient freshwater systems. This study explored variations in composition, richness, evenness and rank-abundance in live and dead mollusk assemblages recovered from the Ibicuí River Basin, southern Brazil. LA/DA was related to topography, river size, and sediment grain size, separated respectively into plain (altitude 0–100 m) versus slope (100–500 m), small versus medium-large stream orders, and gravel versus sand substrate. Positive correlation between LA and DA species composition was significant only in large rivers. Slope areas showed LA/DA species compositions that were significantly different, whereas the communities from sand and gravel substrates were quite similar. Important factors that affected live/dead similarity in the study area included (1) destruction of thin, fragile shells of dead animals by acidic waters that are common in the region, (2) downstream drift of small spherical shells from species common in slope areas, such as Potamolithus sp., and (3) high abundances of invasive species in the local death assemblage, especially in large rivers. High fidelity in large rivers is caused by the presence of favorable habitats for bivalve communities. Coarse sediments are an important driver of macro invertebrate diversity, acting as shell traps that slow the downstream drift of bivalve remains and improve the preservation of fluvial mollusks. The preservation potential of dead assemblages of the Ibicuí River showed that fossil assemblages are useful tools for recognizing ancient riverscapes, such as flat areas with sandy substrates.

In the marine realm, the interpretation of taphofacies relies heavily on how oceanographic and sedimentary conditions affect the preservation state of fossils. Several taphonomic variables either covary with depth or are directly influenced by depth. Facies‐level factors rather than broad, basin‐scale parameters influence the taphonomic profile of mollusc death assemblages according to actualistic and experimental evidence. To determine the possible relation between depth and the taphonomic conditions of multiple species of bivalve remains, we used seven samples gathered over a comprehensive bathymetric gradient (from 7 to 150 m below mean sea level; topmost 10‐ to 20‐cm layer, roughly corresponding to the taphonomically active zone). We selected samples from predominantly muddy facies on the southern Brazilian shelf (SBS). The taphonomic damage profile (TDP) was measured using site samples based on a standard taphonomic analysis (categorical scoring system) of shells and fragments larger than 4 mm, to identify site damage patterns. Restricting the sedimentary grain size (samples from fine sediments) enabled the determination of the variation in damage with depth among the samples. Constrained analysis of proximities (CAP) revealed that up to 46{%} of the taphonomic variation observed was related to variation in depth (with approximately 28{%} unexplained by environmental factors). Part of the unexplained fraction was due to the effect of temporal mixing, which is predictable along large‐scale patches but is inversely linked to the TDP. Our results show that taphonomic analysis, considering large spatial scales in recent environments, can explain the variations present in shell beds that formed during distinct time periods of the evolution of a Quaternary sedimentary basin.

Copyright © 2017, SEPM (Society for Sedimentary Geology). Fossil assemblages are expected to be time-averaged as a result of biological and physical processes that mix skeletal remains. Our quantitative understanding of time-averaging derives primarily from actualistic studies, in which direct numerical dating of individual specimens is used to assess the scale and structure of age mixing in death assemblages (incipient fossil assemblages). Here we examine the age, and the time-averaging of Mactra shells (Bivalvia: Mollusca) gathered from surface mixed siliciclastic-bioclastic sands at three sites on a passive-margin subtropical shelf (the Southern Brazilian Shelf; ∼ 33°S). Sixty Mactra specimens were individually dated using amino acid racemization (AAR) calibrated using radiocarbon ages (n=15). The time-averaging and the total age variability was based on a Bayesian approach that integrates the estimation errors and uncertainties derived from the posterior distribution associated with the AAR calibration average model. The 14C-calibrated AAR ages, pooled across all three sites, are strongly right-skewed with 97{%} of the individual mollusk shell age estimates ranging from 0 to 6 cal kyr BP. The magnitude of time-averaging varied inversely with the water depth, from {\textless} 15 yr at the deepest site (21 m) up to 1020-1250 yr at the shallowest site (7 m). The substantial variation in the temporal resolution across nearby sites, which are located in a seemingly homogenous depositional setting, indicates the presence of notable (if cryptic) spatial heterogeneities in local sedimentation, production, and exhumation, all increasing with water depth.

© 2017 Ochi Agostini et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Empty mollusk shells may act as colonization surfaces for sclerobionts depending on the physical, chemical, and biological attributes of the shells. However, the main factors that can affect the establishment of an organism on hard substrates and the colonization patterns on modern and time-averaged shells remain unclear. Using experimental and field approaches, we compared sclerobiont (i.e., bacteria and invertebrate) colonization patterns on the exposed shells (internal and external sides) of three bivalve species (Anadara brasiliana, Mactra isabelleana, and Amarilladesma mactroides) with different external shell textures. In addition, we evaluated the influence of the host characteristics (mode of life, body size, color alteration, external and internal ornamentation and mineralogy) of sclerobionts on dead mollusk shells (bivalve and gastropod) collected from the Southern Brazilian coast. Finally, we compared field observations with experiments to evaluate how the biological signs of the present-day invertebrate settlements are preserved in molluscan death assemblages (incipient fossil record) in a subtropical shallow coastal setting. The results enhance our understanding of sclerobiont colonization over modern and paleoecology perspectives. The data suggest that sclerobiont settlement is enhanced by (i) high(er) biofilm bacteria density, which is more attracted to surfaces with high ornamentation; (ii) heterogeneous internal and external shell surface; (iii) shallow infaunal or attached epifaunal life modes; (iv) colorful or post-mortem oxidized shell surfaces; (v) shell size ( {\textless} 50 mm2 or {\textgreater} 1,351 mm 2 ); and (vi) calcitic mineralogy. Although the biofilm bacteria density, shell size, and texture are considered the most important factors, the effects of other covarying attributes should also be considered. We observed a similar pattern of sclerobiont colonization frequency over modern and paleoecology perspectives, with an increase of invertebrates occurring on textured bivalve shells. This study demonstrates how bacterial biofilms may influence sclerobiont colonization on biological hosts (mollusks), and shows how ecological relationships in marine organisms may be relevant for interpreting the fossil record of sclerobionts relationships in marine organisms may be relevant for interpreting the fossil record of sclerobionts.

Copyright © 2015, SEPM (Society for Sedimentary Geology). Accurate paleoenvironmental reconstruction relies on the correct interpretation of the postmortem history of skeletal remains in shelly assemblages. In contrast to marine settings, actualistic taphonomic studies are lacking for shell-rich concentrations in freshwater riverine systems. In particular, the taphonomic pathways and the origins of taphonomic signatures that are recorded in bioclasts from fluvial settings are poorly known. In this study, we addressed this issue by comparing the taphonomic signatures and shell-damage profiles among shells of freshwater mollusks recorded both in death and in fossil assemblages from the same fluvial environment. Our data indicated that dissolution was the most pervasive taphonomic process leading to the destruction of the shells. The loss of taphonomic information extended beyond shell dissolution in the riverbed, or the early diagenesis in the sedimentary record. The loss of biological information from the living community through the death assemblage, until the incorporation of shells as fossils, mainly occurred during the time the shells were in the sediment-water interface. Though this destruction affected primarily dead shells, reworked fossils also became vulnerable because they were carried out into the river load again by channel avulsion. A model that included the main taphonomic pathways followed by the molluscan shells in the fluvial Touro Passo Formation (Pleistocene-Holocene) is discussed. In this model, two main destructive domains were recognized, which were the biological, physical, and chemical processes operating at the taphonomically active zone (=TAZ domain) and the pedogenetic domain.

The South America southern coast exhibits many outcrops with abundant shell beds, from the Pleistocene through the Recent. How much biological information is preserved within these shell beds? Or, what is the actual probability a living community has to leave a fossil record corresponding to these shell deposits? Although ecological and biogeographical aspects might had been pointed, considering these temporal scales, up to the moment there is no taphonomically-oriented studies available. Quantitative comparisons between living (LAs), death (DAs) and fossil assemblages (FAs) are important not only in strictly taphonomic studies, but have grown a leading tool for conservation paleobiology analysis. Comparing LAs, DAs and FAs from estuaries and lagoons in the Rio Grande do Sul Coastal Plain makes possible to quantitatively understand the nature and quantity of biological information preserved in fossil associations in Holocene lagoon facies. As already noted by several authors, spatial scale parts the analysis, but we detected that the FAs reflects live ones, rather than dead ones, as previously not realized. The results herein obtained illustrates that species present in DA are not as good preserved in recent (Holocene) fossil record as originally thought. Strictly lagoon species are most prone to leave fossil record. The authors consider that the fidelity pattern here observed for estuarine mollusks to be driven by (i) high temporal and spatial variability in the LAs, (ii) spatial mixing in the DA and (iii) differential preservation of shells, due to long residence times in the taphonomically active zone. © 2013 by the Sociedade Brasileira de Paleontologia.

Molluscan shell accumulations (shell beds) are very common in shallow marine and estuarine environments in South America and also on the continental shelf between Argentina and southern Brazil. The development of these shell beds is restricted to the Quaternary, and can provide a great opportunity for taphonomic studies, which are rare in this geographic location. This study investigates the influence of environmental processes on the destruction of biological remains in a subtropical lagoonal setting by describing the taphonomic signatures occurring in the mollusk shells from the Tramandaí Lagoon, northeastern Rio Grande do Sul State, Brazil. This lagoon system originated during the regressive phase that followed a transgressive maximum at 6.0-6.5 ka BP. The taphonomic variables (fragmentation, margin modification, surface alteration, hinge fine scale alteration and corrosion degree) and their damage states were recorded for 1000 shells of Erodona mactroides. The shells were recovered from two outcrops 8 km apart. 14 C-AMS dating were performed on the shells of E. mactroides from those outcrops, which yielded similar ages (from 1060 to 1490 cal yrs BP). The ages would indicate that the Tramandaí Lagoon occupied a larger area and must have been subjected to very rapid progradation towards its present position. Several taphonomic variables, such as fragmentation, surface alteration, and corrasion extent and oxidation, presented significant differences (p {\textless} 0.01) between the two outcrops. This may be due to some natural variation of the estuarine and lagoonal environments or differences within the taphonomically active zone (TAZ). Dissolution seems to be a leading taphonomic agent in lagoonal environments. The shells from the two outcrops are within the TAZ and, due to intense dissolution, they will most likely leave no geological record. © 2013 Elsevier Ltd and INQUA.

This study is the first assessment of mollusk fossil assemblages relative to the compositional fidelity of modern mollusk living and death assemblages. It also shows that the sedimentary record can provide information on the original, non-human-impacted, freshwater malacofauna biodiversity, based on Late Pleistocene shells. The fossil mollusk assemblage from the Touro Passo Formation (Pleistocene-Holocene) was compared to living and death assemblages of the Touro Passo River, southern Brazil, revealing little resemblance between fossil and live-dead species composition. Although the living and death assemblages agree closely in richness, species composition, and species relative abundances (both proportional and rank), the fossil assemblage differs significantly from both modern assemblages in most of these measures. The fossil assemblage is dominated by the native endemic corbiculid bivalve Cyanocyclas limosa and the gastropod Heleobia aff. bertoniana. These are absent in the living assemblages, and both living and death assemblages are dominated by the alien Asiatic corbiculid C. fluminea, which is absent in the fossil assemblage. The fossil assemblage also contains, overall, a higher proportional abundance of relatively thick-shelled species, suggesting a genuine bias against the thinner-and smaller-shelled species. Our results suggest that contemporary environmental changes, such as the introduction of some alien freshwater mollusk species, together with post-burial taphonomic processes, are the main factors leading to the poor fidelity of the fossil assemblage stu died. Hence, the taxonomic composition of the Late Pleistocene mollusks from the Touro Passo Formation probably would show greater similarity to present-day assemblages wherever the mollusk biodiversity is not disturbed by human activities. © 2011 SEPM (Society for Sedimentary Geology).

In this work was investigated the effect of pre-treatment with (PhSe) 2 and (PhTe) 2 on chemical seizure and 4-aminopyridine-induced lethality in mice. Additionally, lipid peroxidation levels of whole brain after treatment with 4-aminopyridine and effect of pre-treatment with (PhSe) 2 and (PhTe) 2 on these levels were investigated. Mice were pre-treated with (PhSe) 2 or (PhTe) 2 (50, 100, or 150 $μ$mol/kg) 30 min before 4-aminopyridine (12 mg/kg) administration. The treatment with 4-aminopyridine caused a significant incidence of seizures (clonic, tonic) and death. Pre-treatment with (PhSe) 2 and (PhTe) 2 significantly increased the latency for clonic and tonic seizures, and prevented 4-aminopyridine-induced death. Significantly, the pre-treatment with (PhSe) 2 or (PhTe) 2 increased the latency for clonic seizures in a dose-dependent manner. Additionally, a significant increase was observed in the brain lipid peroxidation level after treatment with 4-aminopyridine, which was significantly inhibited by pre-treatment with 150 $μ$mol/kg (PhSe) 2 or (PhTe) 2 . These results demonstrate that (PhSe) 2 and (PhTe) 2 counteract the harmful effects of 4-aminopyridine. It is possible that this effect results from modulation of the redox state of N-methyl-d-aspartate receptors and/or of Ca 2+ channel activity with subsequent alteration in neurotransmitter release. Importantly, this study provides evidence for anticonvulsant and antioxidant properties of (PhSe) 2 and (PhTe) 2′ which indicates a neuroprotective activity of these compounds.

Objectives: To evaluate the potential role of oxidative stress in the evolution of cervical cancer, including its pre-malignant states. Design and methods: Erythrocytes thiobarbituric acid reactive substances (TBARS) levels, plasma vitamin C and thiol content and total blood $δ$-ALA-D levels were estimated in 46 untreated cervical cancer and pre-malignant patients and in 46 age-sex-matched controls. Results: Erythrocytes from patients, regardless of disease state, pre-malignant (low squamous intraepithelial lesion-LSIL and high squamous intraepithelial lesion-HSIL) or cancer, showed a significant 2-3 times increase in TBARS levels (P {\textless} 0.01). Plasma vitamin C was lower in the carcinoma group (P {\textless} 0.01). The reactivation index of $δ$-aminolevulinate dehydratase ($δ$-ALA-D) was higher in the patient group, when compared to control (P {\textless} 0.01). Conclusion: LSIL, HSIL or cervical cancer can be associated with changes in 3 indicators of oxidative stress: increase in erythrocyte TBARS, ALA-D reactivation index and a decrease in vitamin C content, that may play an important role in carcinogenesis. © 2005 The Canadian Society of Clinical Chemists. All rights reserved.